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KMID : 0357920100440030225
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Korean Journal of Pathology
2010 Volume.44 No. 3 p.225 ~ p.233
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Validation of Gene Expression Changes of Osteopontin and MMP-1 in Primary and Metastatic Colorectal Carcinomas
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Choi Jun-Jeong
Kim Sang-Kyum
Park Jeon-Han
Kim Nam-Kyu
Kim Ho-Guen
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Abstract
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Background: Metastasis is one of the most important characteristics of cancer in terms of its impact on patient survival. Unfortunately, identification of altered genes during tumor metastasis is limited.
Methods: Using high-throughput microarrays containing 19K spotted human oligonucleotides, gene expression of primary and matched metastatic colon cancer were compared in previous study. Although DNA microarray analysis did not demonstrate complete classification of primary and metastatic carcinoma, 80 differentially expressed genes were identified. Among these, expression of osteopontin, matrix metalloproteinase-1 (MMP-1) and serpin A1 was assessed using immunohistochemistry in a validation set containing 43 pairs from tissue microarrays.
Results:
Conclusions: Osteopontin and MMP-1 expression successfully classified primary and metastatic colorectal carcinomas and further studies on their clinical application is encouraged.
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KEYWORD
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Neoplasm metastasis, Colorectal neoplasms, Immunohistochemistry, Osteopontin, Matrix metalloproteinase 1
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